What is DEAE Sepharose chromatography?
DEAE Sepharose Fast Flow is a weak anion exchange chromatography resin (medium) for fast protein purification. Well-proven weak anion exchanger developed for industrial downstream processes. Used extensively for capture and intermediate purification of a wide range of approved biopharmaceuticals.
What is a DEAE column?
SMT DEAE [Di-Ethyl-Amino-Ethyl] column provides a unique chemically attached hydrophilic, weak anion exchange type, functional surface desirable for the separation of many biomolecules such as proteins, nucleotides, oligonucleotides, polynucleotides, high molecular weight RNA’s and plasmid DNA’s.
What is the meaning of DEAE?
to the goddess, goddess, goddesses.
Is DEAE cellulose an anion or cation exchanger?
weak anion exchanger
DEAE-cellulose contains an diethylaminoethyl group. It is positively charged at neutral pH and so DEAE-cellulose is a weak anion exchanger. The Sepharose types are particularly useful for the separation of high molecular weight proteins.
Is DEAE a cation exchanger?
At neutral pH the carboxymethyl group is ionized as -CH2OCH2COO¯ so that CM- cellulose is negatively charged, so it is a weak cation exchanger. DEAE-cellulose contains an diethylaminoethyl group. It is positively charged at neutral pH and so DEAE-cellulose is a weak anion exchanger.
Why cellulose paper is used in paper chromatography?
Principle of Paper chromatography Substances are distributed between a stationary phase and mobile phase. Cellulose layers in filter paper contain moisture which acts as stationary phase. The developing solution travels up the stationary phase carrying the sample with it.
What is the charge of DEAE cellulose?
What is the nature of the ion exchanger in DEAE?
DEAE Sephacel™ is a weak anion exchanger based on beaded cellulose. The ion exchange group is diethylaminoethyl, which remains charged and maintains consistently high capacity over the entire working range, pH 2 to 9.
Why is cellulose used in chromatography?
Cellulose chromatography paper provides a novel non-covalent support for synthesis and in-situ purification of multi-dimensional arrays.